Cellulases : Methods and Protocols - Lübeck, Mette; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Cellulases: Role in Lignocellulosic Biomass Utilization -- Discovery of a Novel Fungus with an Extraordinary b-Glucosidase -- Discovery of New Cellulases Using Classical or Molecular Methods -- Isolation and Screening of Cellulolytic Filamentous Fungi -- Isolation and Screening of Cellulose Degrading Microorganisms from Different Ecological Niches -- Isolation of Cellulolytic Bacteria from the Rumen -- Methods for Discovery of Novel Cellulosomal Cellulases using Genomics and Biochemical Tools -- Metatranscriptomic Techniques for Identifying Cellulases in Termites and Their Symbionts -- Discovery of Novel Cellulases Using Proteomic Strategies -- Identification of Key Components for the Optimization of Cellulase Mixtures Using a Proteomic Strategy -- Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger -- Advanced Cloning Tools for Construction of Designer Cellulosomes -- Evaluation of Thermal Stability of Cellulosomal Hydrolases and Their complex Formation -- Endoglucanase (EG) Activity Assays -- Cellobiohydolase (CBH) Activity Assays -- Two-Dimensional High-Throughput Endo-Enzyme Screening Assays Based on Chromogenic Polysaccharide Hydrogel and Complex Biomass Substrates -- Analytical Tools for Characterizing Cellulose-Active Lytic Polysaccharide Monooxygenases (LPMOs) -- Light Induced Electron Transfer Protocol for Enzymatic Oxidation of Polysaccharide -- Purification of Cellulase from Cellulolytic Microorganisms and Characterization of the Total Cellulase Activities (TCA) -- Fungal On-Site Production of Cellulolytic Enzymes by the Sequential Cultivation Method -- Test of Efficacy of Cellulases for Biomass Degradation -- Homology Modeling for Enzyme Design.
Keywords: Biochemistry; Biochemistry, general