Autophagy : Methods and Protocols - Ktistakis, Nicholas; Florey, Oliver; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Recombinant Expression, Purification and Assembly of p62 Filaments -- Structural Studies of Autophagy-related Proteins -- Structural Studies of Mammalian Autophagy Lipidation Complex -- Structural Studies of Selective Autophagy in Yeast -- Biophysical Studies of LC3 Family Proteins -- Reconstituting Autophagy Initiation from Purified Components -- Cell-free Reconstitution of Autophagic Membrane Formation -- Use of Peptide Arrays for Identification and Characterization of LIR Motifs -- Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution System -- Formation of Autophagosomes Coincides With Relaxation of Membrane Curvature -- Studies of Receptor-Atg8 Interactions during Selective Autophagy -- Correlative Light and Electron Miscopy of Autophagosomes -- Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian Cells -- 3-color Simultaneous Live Imaging of Autophagy-related Structures -- Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy -- Methods for Imaging Autophagosome Dynamics in Primary Neurons -- Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research -- Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryo -- Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured Cells -- Measurement of Bulk Autophagy by a Cargo Sequestration Assay -- Methods to Detect Loss of Lysosomal Membrane Integrity -- Monitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress -- Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry Approaches -- Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME Screen -- Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput Screening -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells -- Methods for the Study of Entotic Cell Death -- MHC Class I Internalization via Autophagy Proteins -- Analysis of Autophagy for Liver Pathogenesis -- Autophagy in 3D In Vitro and Ex Vivo Cancer Models -- Autophagy in Platelets -- Methods to Image Macroautophagy in the Brain In Vivo -- Measuring Nonselective and Selective Autophagy in the Liver -- Measuring Autophagy in Pancreatitis -- Characterization of the "autophagic flux" in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy -- Methods to Determine the Role of Autophagy Proteins in C. elegans Ageing -- Investigating Non-selective Autophagy in Drosophila -- Imaging the Dynamics of Mitophagy in Live Cells -- Triggering Mitophagy with Photosensitizers -- Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QC -- Assays to Monitor Mitophagy in Drosophila -- Mitophagy Dynamics in Caenorhabditis elegans -- Methods for Studying Mitophagy in Yeast -- Measuring Anti-bacterial Autophagy -- Quantitative Phosphoproteomics of Selective Autophagy Receptors -- Analysis of Chaperone-mediated Autophagy -- Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic Observations.
Keywords: Biochemistry; Biochemistry, general