Calpain : Methods and Protocols - Messer, Jeannette S.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Springer, 2019)
Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin using Anion Exchange Chromatography -- Bacterial Expression and Purification of Calpains -- Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity -- FRET-Based Assays to Determine Calpain Activity -- In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer -- Determining Temporal and Spatial Expression of Calpains in Amphibians -- Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis -- Expression and Activity of Calpain A in Drosophila melanogaster -- Use of the β-Glucoronidase Reporter System (GUS) to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1) -- LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence -- CalCleaveMKL: A Tool for Calpain Cleavage Prediction -- Identification of Calpain-Activated protein Functions -- Production and Purification of Recombinant Calpastatin -- Isolation of Endogenous Calpastatin -- Immunoblotting for Calpastatin Expression -- Experimental Manipulation of Calpain Activity In Vitro -- In Vivo Calpain Knockdown using Delivery of siRNA -- In Vivo Expression of Mutant Calpains in the Eye using Lentivirus -- Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians -- Genetic Models of Calpain Deficiency and Ectopic Expression.
Keywords: Proteins .; Protein Science