Bone Research Protocols - Idris, Aymen I.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Isolation and Generation of Osteoclasts -- Isolation and Generation of Osteoblasts -- Isolation, Purification, Generation, and Culture of Osteocytes -- Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo -- 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies -- Using Cell and Organ Culture Models to Analyse Responses of Bone Cells To Mechanical Stimulation -- The ex vivo Organ Culture of Bone -- Ex-Vivo Model Systems of Cancer-bone Cell Interactions -- Mechanical Properties of Bone ex vivo -- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation -- Analysis of Transcriptional Regulation in Bone Cells -- Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization -- Ovariectomy/Orchiectomy in Rodents -- In Vivo Models of Rheumatoid Arthritis -- Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus -- Models of Prostate Cancer Bone Metastasis -- Animal Models of Breast Cancer Bone Metastasis -- Murine Models of Bone Sarcomas -- Mouse Models of Melanoma Bone Metastasis -- Animal Models of Multiple Myeloma -- The Calvarial Injection Assay -- In Vivo Models of Mechanical Loading -- Animal Models for the Study of Bone Derived Pain -- Histomorphometry in Rodents -- Immunostaining of Skeletal Tissues -- Techniques for the Study of Apoptosis in Bone -- Live Cell Imaging of Bone Cell and Organ Cultures -- Analysis of Bone Architecture in Rodents using micro Computed Tomography -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- Quantitative X-ray Imaging of Mouse Bone by Faxitron -- Scanning Electron Microscopy of Bone -- Transmission Electron Microscopy of Bone -- Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy -- Fourier Transform Infrared Imaging of bone -- Raman Microscopy and Bone.
Keywords: Molecular biology; Orthopedics; Molecular Medicine; Orthopedics