Antibody Engineering : Methods and Protocols - Nevoltris, Damien; Chames, Patrick; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Antibody Design and Humanization via In Silico Modeling -- Antibody Affinity Maturation by Computational Design -- Use of IMGT® Databases and Tools for Antibody Engineering and Humanization -- Construction of Human Naïve Antibody Gene Libraries -- Construction of Synthetic Antibody Libraries -- Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments -- Display Technologies for Generation of Ig Single Variable Domains -- A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries -- Phage Display and Selections on Purified Antigens -- Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display -- Selection of Antibody Fragments against Structured DNA by Phage Display -- Selection of Antibody Fragments by Yeast Display -- Rapid Selection of High Affinity Antibody scFv Fragments Using Ribosome Display -- In Vitro Selection of Single Domain Antibody (VHH) Using cDNA Display -- Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood -- Expression of IgG Monoclonals with Engineered Immune Effector Functions -- An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High Level Monoclonal Antibody Producing CHO DG44 Cell Lines -- Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins -- Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases -- Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity -- Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High Affinity Interaction with FcRn at pH 6.0 -- Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance -- Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development -- The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies -- Selection and Use of Intracellular Antibodies -- Site-Specific Radioactive Labeling of Nanobodies.
Keywords: Immunology; Immunology