Cancer Immunosurveillance : Methods and Protocols - López-Soto, Alejandro; Folgueras, Alicia R.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer -- An Economical, Quantitative, and Robust Protocol for High Throughput T Cell Receptor Sequencing from Tumor or Blood -- Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells -- Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Anti-Tumor Effects In Vivo -- Usage of Single-Cell RNA-Sequencing to Unveil Immune Lymphoid Cell Precursors -- Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance -- CD107a Degranulation Assay to Evaluate Immune Cell Anti-Tumor Activity -- A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anti-Cancer Immune Responses In Vitro -- Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency -- Tumor-Associated Macrophage Isolation and In Vivo Analysis of their Tumor Promoting Activity -- Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo -- Determination and Isolation of Immune Populations from Brain Tumor Microenvironments -- Mouse Model of Colitis Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells -- Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry -- Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways -- Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation -- Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion -- Quantitative Identification of Senescent Cells in Cancer -- Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance -- Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment -- Gold Standard Assessment of Immunogenic Cell Death in Oncological Mouse Models -- A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers -- Development of Bi-Specific Antibody Derivatives for Cancer Immunotherapy -- Generation of CAR-T Cells for Cancer Immunotherapy.
Keywords: Cancer research; Cancer Research