Alpha-Synuclein : Methods and Protocols - Bartels, Tim.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons -- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue -- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons -- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform -- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils -- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection -- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers -- Studying α-Synuclein Conformation by Intact-Cell Crosslinking -- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques -- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein -- Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes -- Yeast-Based Screens to Target Alpha-Synuclein Toxicity -- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy -- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers -- Monitoring α-Synuclein Proteotoxicity in Drosophila Models -- Detection of α-Synuclein in Biological Samples using Mass Spectrometry -- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation -- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons -- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy -- Expression and Purification of Untagged α-Synuclein -- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson's Disease.
Keywords: Proteins .; Neurosciences; Protein Science; Neurosciences