BCL-2 Family Proteins : Methods and Protocols - Gavathiotis, Evripidis; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Overview of BCL-2 Family Proteins and Therapeutic Potentials -- Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins -- Application of Mito-Priming to Generate BCL-2 Addicted Cells -- BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies -- Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP) -- Investigating BCL-2 Family Protein Interactions in Yeast -- Liposomal Permeabilization Assay to Study the Functional Interaction of the BCL-2 Family -- Mitochondrial Isolation, Gel Filtration, and Real-Time Monitoring of MOMP -- Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family -- Assessment of Dynamic BCL-2 Protein Shuttling between Outer Mitochondrial Membrane and Cytosol -- Quantification of BCL-2 Family Members by Flow Cytometry -- Methods to Probe Calcium Regulation by BCL-2 Family Members -- Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK -- Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis and Oxidant-Induced Linkage -- BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy -- Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs -- Cryo-Electron Microscopy to Study Bax Pores and MOMP -- CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores -- Rapid Imaging of BCL-2 Family Interactions in Live Cells using FLIM-FRET -- Quantification of the Interactions between BCL-2 Proteins by Fluorescence Correlation Spectroscopy -- Isolation of Synthetic Antibodies against BCL-2-Associated X Protein (BAX).
Keywords: Proteins .; Protein Science