Cell-Based Assays for High-Throughput Screening : Methods and Protocols - Clemons, Paul A.; Tolliday, Nicola J.; Wagner, Bridget K.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Introduction: Cell-Based Assays for High-Throughput Screening -- High-Throughput Screening of Model Bacteria -- Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells -- Large-Scale Small-Molecule Screen Using Zebrafish Embryos -- Whole-Animal High-Throughput Screens: The C. elegans Model -- Whole-Organism Screening: Plants -- Fluorescence-Based Assays -- Reporter Gene Assays -- Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-Restoration Assay -- Assay for Isolation of Inhibitors of Her2-Kinase Expression -- High-Content Screening: Flow Cytometry Analysis -- High-Throughput Real-Time PCR for Detection of Gene-Expression Levels -- Interpretation of Uniform-Well Readouts -- Extracting Rich Information from Images.
Keywords: Biochemistry; Combinatorial chemistry; Biochemistry, general; Combinatorial Libraries