Breast Cancer Research Protocols - Brooks, Susan A.; Harris, Adrian; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2006)
Preparation of Cell and Tissue Samples -- Breast Tissue Banking -- Frozen Biopsy Collection and Storage -- Serial Biopsies/Fine-Needle Aspirates and Their Assessment -- Breast Tissue Microarrays -- Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA -- Isolation of RNA From Tumor Samples -- Isolation of DNA From Tumor Samples -- Laser-Assisted Microdissection and Isolation of DNA and RNA -- Markers of Clinical Outcome and Prognosis -- Traditional and Established Indicators of Prognosis and Treatment Success -- Sentinel Lymph Node Biopsy in Breast Cancer -- Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer -- Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections -- Markers of Apoptosis -- Quantitative Angiogenesis in Breast Cancer -- Analysis of Tumor-Derived Proteins and Antigens -- Immunohistochemistry -- Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry -- SDS-PAGE and Western Blotting to Detect Proteins and Glycoproteins of Interest in Breast Cancer Research -- Breast Cancer Proteomics Using Two-Dimensional Electrophoresis -- Procedures for the Quantitative Protein Determination of Urokinase and Its Inhibitor, PAI-1, in Human Breast Cancer Tissue Extracts by ELISA -- Analysis of Genes and Gene Expression in Tumor Specimens -- Fluorescence In Situ Hybridization and Comparative Genomic Hybridization -- Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues -- Detection of HER2 Gene Amplification by Fluorescence In Situ Hybridization in Breast Cancer -- In Situ Hybridization Combined With Immunohistochemistry to Localize Gene Expression -- Quantitation of RNA by Ribonuclease Protection Assay -- Identification of Steroid Hormone-Regulated Genes in Breast Cancer -- Sequencing of the Tumor Suppressor Gene TP 53 -- Expression Profiling Using cDNA Microarrays -- Gene Expression Analysis Using Filter cDNA Microarrays -- Studying Cancer Cell Behavior in Vitro and In Vivo -- Methods to Analyze the Effects of the Urokinase System on Cancer Cell Adhesion, Proliferation, Migration, and Signal Transduction Events -- Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage -- Models of Hormone Resistance In Vitro and In Vivo -- Generation of Genetically Modified Embryonic Stem Cells for the Development of Knockout Mouse Animal Model Systems -- In Vivo Xenograft Models of Breast Cancer Metastasis -- Neoadjuvant Endocrine Therapy Models -- Primary Mouse Endothelial Cell Culture for Assays of Angiogenesis.
Keywords: Oncology .; Oncology