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03534nam a22003375i 4500 |
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20200922084238.0 |
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cr nn 008mamaa |
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190312s2019 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493991549
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| 024 |
7 |
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|a 10.1007/978-1-4939-9154-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Bacterial Polysaccharides :
|b Methods and Protocols /
|c edited by Inka Brockhausen.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XIII, 312 p. 70 illus., 41 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1954
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| 505 |
0 |
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|a 1. Customisable cloning of whole polysaccharide gene clusters by yeast homologous recombination -- 2. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-negative Bacteria -- 3. Purification of Capsular Polysaccharide Complex from Gram Negative Bacteria -- 4. Simple protocol to purify cell wall polysaccharide from Gram+ bacteria and assess its structural integrity -- 5. Extraction and purification of wall bound polymers of Gram-positive bacteria -- 6. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan and Lipoarabinomannan -- 7. Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry -- 8. Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry -- 9. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for studies in cytokine production -- 10. O-Acetylation of Bacterial Cell Wall Polysaccharides -- 11. Designing glycosyltransferase expression constructs for improved purification, protein yield and crystallization -- 12. Utilization of fluorescently-tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, KpsC, from Escherichia coli -- 13. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O-Antigenic Polysaccharides in Enteric Bacteria -- 14. Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides -- 15. Enzymatic synthesis of the repeating unit oligosaccharides of Escherichia coli O104 -- 16. Sulfhydryl labelling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis -- 17. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides -- 18. Glycosyltransferase Activity Assays Using Colorimetric Methods -- 19. In vitro characterization of a multidomain glycosyltransferase using fluorescently-tagged synthetic acceptors -- 20. Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa -- 21. Assaying fucosidase activity -- 22. In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis -- 23. High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus.
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| 650 |
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0 |
|a Bacteriology.
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| 650 |
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0 |
|a Enzymology.
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| 650 |
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0 |
|a Immunology.
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| 650 |
1 |
4 |
|a Bacteriology.
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| 650 |
2 |
4 |
|a Enzymology.
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| 650 |
2 |
4 |
|a Immunology.
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| 700 |
1 |
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|a Brockhausen, Inka.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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