CRISPR Gene Editing : Methods and Protocols - Luo, Yonglun; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
CRISPR-gRNA Design -- Tracking CRISPR's Footprints -- Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER -- Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA -- Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check -- CRISPR-Cas9 Delivery by Artificial Virus (RRPHC) -- Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery -- Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors -- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs -- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 -- Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes -- Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9 -- CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells -- Rapid and Efficient Gene Deletion by CRISPR/Cas9 -- Genome Editing in Mice -- CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol -- Gene Editing in Primary Cells of Cattle and Pig -- Toward In Vivo Gene Therapy Using CRISPR -- CRISPR Gene Therapy of the Eye: Targeted Knockout of Vegfa in Mouse Retina by Lentiviral Delivery -- In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection -- CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes.
Keywords: Human genetics; Genetic engineering; Human Genetics; Genetic Engineering