Beta-Arrestins : Methods and Protocols - Scott, Mark G. H.; Laporte, Stéphane A.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
A Brief History of the b-Arrestins -- b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling -- Methods to Monitor the Trafficking of β-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET -- Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle -- Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer -- Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET -- Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin -- Methods to Investigate Arrestins in Complex with Phosphodiesterases -- Methods to Characterize Protein Interactions with β-arrestin in cellulo -- Methods to Investigate the β-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling -- Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing -- Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners -- Workflow Description to Dynamically Model β-Arrestin Signaling Networks -- Proteomic Analysis of the β-Arrestin Interactome -- Quantitating Ligand Bias using the Competitive Model of Ligand Activity -- Methods to Investigate the Nucleocytoplasmic Shuttling Properties of b-Arrestins -- Monitoring β-Arrestin2 Targeting to the Centrosome, Basal Body and Primary Cilium by Fluorescence Microscopy -- A Mass Spectrometry-Based Structural Assay for Activation-Dependent Conformational Changes in β-Arrestins -- Probing Arrestin Function using Intramolecular FlAsH-BRET Biosensors -- Methods to Study the Roles of b-Arrestins in Meningococcal Signaling -- Methods to Investigate the Roles for β-Arrestin2 in Allergic Inflammatory Airway Disease -- Methods to Study Roles of β-Arrestins in the Regulation of Pancreatic β-Cell Function -- Methods to Investigate β-Arrestin Function in Metabolic Regulation -- Methods to Investigate the Role of b-Arrestin Signaling in Parkinson's Ddisease -- Methods to Investigate β-Arrestin-1/β-Catenin Signaling in Ovarian Cancer Cells.
Keywords: Proteins .; Cell biology; Protein Science; Cell Biology; Receptors