Bacterial Regulatory RNA : Methods and Protocols - Arluison, Véronique.; Valverde, Claudio; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Workflow for a Computational Analysis of an sRNA Candidate in Bacteria -- Molecular Phylogenomics for the Identification and Characterization of trans-encoded Small Non-coding RNA Families -- Bioinformatic Approach for Prediction of CsrA/RsmA-regulating small RNAs in Bacteria -- Host-pathogen Transcriptomics by Dual RNA-seq -- Identification of New Bacterial Small RNA Targets using MS2 Affinity Purification Coupled to RNA Sequencing -- Assessment of External Guide Sequences' (EGS) Efficiency as Inducers of RNase P-mediated Cleavage of mRNA Target Molecules -- Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-dependent Termination of Transcription in vitro -- A Method to Map Changes in Bacterial Surface Composition Induced by Regulatory RNAs -- Fluorescence-based Methods for Characterizing RNA interactions in vivo -- Mutational Analysis of sRNA-mRNA Base Pairing by Electrophoretic Mobility Shift Assay -- An Integrated Cell-free Assay to Study Translation Regulation by Small Bacterial Non-coding RNAs -- Quantitative Super-resolution Imaging of Small RNAs in Bacterial Cells -- Extraction and Analysis of RNA Isolated from Pure Bacteria-Derived outer Membrane Vesicles -- Absolute Regulatory Small Noncoding RNA Concentration and Decay Rates Measurements in Escherichia coli -- High-resolution, High-throughput Analysis of Hfq Binding Sites using UV-crosslinking and Analysis of cDNA (CRAC) -- Producing Hfq/Sm Proteins and sRNAs for Structural and Biophysical Studies of Ribonucleoprotein Assembly -- Single Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing -- Techniques to Analyse sRNA-protein Cofactor Self-assembly in vitro -- Sequence-specific Affinity Chromatography of RNA-binding Proteins from Bacterial Cells -- Identification of Small RNA-protein Partners in Plant Symbiotic Bacteria -- A Modular Genetic System for High-throughput Profiling and Engineering of Multi-target Small RNAs. .
Keywords: Human genetics; Human Genetics