Affinity Chromatography : Methods and Protocols - Zachariou, Michael; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Preface -- Contributors -- Affinity Chromatography: History, Perspectives, Limitations and ProspectsAna Ceclia A. Roque and Christopher R. Lowe 1 -- Various Modes of Affinity Chromatography -- Immobilized Metal Ion Affinity Chromatography of Native ProteinsAdam Charlton and Michael Zachariou -- Affinity Precipitation of Proteins Using Metal ChelatesAshok Kumar, Igor Yu. Galaev, and Bo Mattiasson -- Immunoaffinity ChromatographyStuart R. Gallant, Vish Koppaka, and Nick Zecherle -- Dye Ligand ChromatographyStuart R. Gallant, Vish Koppaka, and Nick Zecherle -- Purification of Proteins Using Displacement ChromatographyNihal Tugcu -- Affinity Chromatography Using Purification Tags -- Rationally Designed Ligands for Use in Affinity Chromatography: An Artificial Protein LAna Ceclia A. Roque and Christopher R. Lowe -- Phage Display of Peptides in Ligand Selection for Use in Affinity ChromatographyJoanne L. Casey, Andrew M. Coley, and Michael Foley -- Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion ProteinGareth M. Forde -- Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion ProteinsAdam Charlton and Michael Zachariou -- Methods for the Purification of HQ-Tagged ProteinsBecky Godat, Laurie Engel, Natalie A. Betz, and Tonny M. Johnson -- Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Matrix-Assisted Dialysis Refolding MethodsLeonard K. Pattenden and Walter G. Thomas -- Methods for Detection of Protein-Protein and Protein-DNA Interactions Using HaloTagMarjeta Urh, Danette Hartzell, Jacqui Mendez, Dieter H. Klaubert, and Keith Wood -- Site-Specific Cleavage of Fusion ProteinsAdam Charlton -- The Use of TAGZyme for the Efficient Removal of N-Terminal His-TagsJose? Arnau, Conni Lauritzen, Gitte Ebert Petersen, and John Pedersen -- Various Applications of Affinity Chromatography -- Affinity Processing of Cell-Containing Feeds Using Monolithic Macroporous Hydrogels, CryogelsIgor Yu. Galaev and Bo Mattiasson -- Monolithic Bioreactors for MacromoleculesMojca Ben?ina, Katja Ben?ina, Aleš Podgornik, and Aleš Štrancar -- Plasmid DNA Purification Via the Use of a Dual Affinity ProteinGareth M. Forde -- Affinity Chromatography of Phosphorylated ProteinsGrigoriy S. Tchaga -- Protein Separation Using Immobilized Phospholipid ChromatographyTzong-Hsien Lee and Marie-Isabel Aguilar -- Analysis of Proteins in Solution Using Affinity Capillary ElectrophoresisNiels H. H. Heegaard, Christian Schou, and Jesper Østergaard.
Keywords: Cell biology; Human genetics; Biotechnology; Pharmacology; Cell Biology; Human Genetics; Biotechnology; Pharmacology/Toxicology.