Cell Cycle Checkpoint Control Protocols - Lieberman, Howard B.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Induction and Detection of Changes in cell Cycle Progression -- Methods to Induce Cell Cycle Checkpoints -- Methods for Synchronizing Mammalian Cells -- Enrichment of Cells in Different Phases of the Cell Cycle by Centrifugal Elutriation -- Analysis of the Mammalian Cell Cycle by Flow Cytometry -- Methods for Detecting Cells in S Phase -- Yeast Cell Synchronization -- Analysis of the Budding Yeast Saccharomyces cerevisiae Cell Cycle by Morphological Criteria and Flow Cytometry -- Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle -- Analysis of Genes Involved in Checkpoint Control -- Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes -- Microarray Approaches for Analysis of Cell Cycle Regulatory Genes -- Using the Yeast Genome-Wide Gene-Deletion Collection for Systematic Genetic Screens -- Gene Targeting in Cultured Human Cells -- Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene -- In Vitro Mutagenesis to Define Functional Domains -- Use of Gene Overexpression to Assess Function in Cell Cycle Control -- Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression -- Analysis of Proteins Involved in Checkpoint Control -- Cataloging Proteins in Cell Cycle Control -- Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control -- Purification and Identification of Protein Complexes That Control the Cell Cycle -- Xenopus Cell-Free Extracts to Study DNA Damage Checkpoints -- Protein-Protein Interactions -- Detection of Kinase and Phosphatase Activities -- Monitoring Changes in the Subcellular Location of Proteins in S. cerevisiae -- Chromosomes and the Cell Cycle -- Chromosomal Changes and Cell Cycle Checkpoints in Mammalian Cells -- Detecting the Influence of Cell Cycle Regulatory Proteins on Human Telomeres -- Monitoring Spindle Assembly and Disassembly in Yeast by Indirect Immunofluorescence.
Keywords: Cell biology; Cell Biology