Bacterial Artificial Chromosomes : Volume 1: Library Construction, Physical Mapping, and Sequencing /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
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| Edición: | 1st ed. 2004. |
| Colección: | Methods in Molecular Biology,
255 |
| Materias: |
Tabla de Contenidos:
- BAC Library Construction
- Construction of Small Genome BAC Library for Functional and Genomic Applications
- Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging
- Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions
- Purification of BAC DNA
- Hybridization-Based Selection of BAC Clones
- Applications of Interspersed Repeat Sequence Polymerase Chain Reaction
- BAC Mapping Using Fluorescence In Situ Hybridization
- High-Throughput BAC Fingerprinting
- BAC End Sequencing
- Radiation Hybrid Mapping With BAC Ends
- Shotgun Library Construction for DNA Sequencing
- Rolling Circle Amplification for Sequencing Templates
- Transposon-Mediated Sequencing
- Pyrosequencing
- Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions
- Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions
- Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers
- BAC Finishing Strategies
- Using the TIGR Assembler in Shotgun Sequencing Projects
- Finishing “Working Draft” BAC Projects by Directed Sequencing With ThermoFidelase and Fimers
- Optimized Multiplex Polymerase Chain Reaction
- Assembly of DNA Sequencing Data
- Sequence Finishing
- Quality Assessment of Finished BAC Sequences.