Cartilage and Osteoarthritis - Sabatini, Massimo; Pastoureau, Philippe; De Ceuninck, FrTdTric; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Culture and Phenotyping of Chondrocytes in Primary Culture -- Culture of Chondrocytes in Alginate Beads -- Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines -- Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function -- Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype -- Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR -- Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies -- RNA Extraction From Cartilage -- Gene Expression Analysis in Cartilage by In Situ Hybridization -- Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis -- High-Efficiency Nonviral Transfection of Primary Chondrocytes -- In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors -- Changes of Chondrocyte Metabolism In Vitro -- Analysis of Chondrocyte Functional Markers and Pericellular Matrix Components by Flow Cytometry -- A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes -- Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants -- Production of Antibodies Against Degradative Neoepitopes in Aggrecan -- Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures -- Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes -- Expression, Activity, and Regulation of MAP Kinases in Cultured Chondrocytes -- Mechanical Loading of Chondrocytes Embedded in 3D Constructs -- In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage.
Keywords: Endocrinology .; Endocrinology