Chromatin Protocols - Chellappan, Srikumar P.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos -- Analysis of Reconstituted Chromatin Using a Solid-Phase Approach -- In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins -- DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules -- Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues -- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin -- Analysis of DNA Topology in Yeast Chromatin -- Preparation and Analysis of Uniquely Positioned Mononucleosomes -- Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy -- Methods to Study Transcription-Coupled Repair in Chromatin -- Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs) -- Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay -- Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes -- In Vitro Replication Assay with Mammalian Cell Extracts -- Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polymerase Chain Reaction -- Biochemical Analysis of Arginine Methylation in Transcription -- In Vitro Histone Demethylase Assays -- Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation -- Histone Deacetylase Activity Assay -- In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination -- Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining -- Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo -- ChIP on Chip Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications -- ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays -- Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length -- Detecting ATM-Dependent Chromatin Modification in DNA Damage and Heat Shock Response.
Keywords: Biochemistry; Nucleic acids; Gene expression; Biochemistry, general; Nucleic Acid Chemistry; Gene Expression