Cell-Penetrating Peptides : Methods and Protocols - Langel, Ülo.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Classes and prediction of cell-penetrating peptides -- Penetratin story : an overview -- Testing membrane interactions of CPPs -- Interactions of amphipathic CPPs with model membranes -- NMR studies of three-dimensional structure and positioning of CPPs in membrane model systems -- Measurements of the intracellular stability of CPPs -- Tools for predicting binding and insertion of CPPs into lipid bilayers -- Studies of proteoglycan involvement in CPP-mediated delivery -- Uptake Kinetics of cell-penetrating peptides -- Thermodynamics of lipid interactions with cell-penetrating peptides -- Calcium and membrane repair -- Mapping of protein transduction pathways with fluorescent microscopy -- Insight into cell-entry mechanisms of CPPs by electron microscopy -- Toxicity methods for CPPs -- Comparison of CPP uptake methods -- Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery -- Mimicry of protein function with cell-penetrating peptides -- Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptides -- Pharmacology, biodistribution, and efficacy of GPCR-based pepducins in disease models -- Identification and Characterization of Tissue-Specific Protein Transduction Domains using Peptide Phage Display -- Applications of Cell Penetrating Peptides as Signal Transduction Modulators for the Selective Induction of Apoptosis -- Splice Redirection as a Convenient Assay to Monitor CPP-ON Efficiency and Mechanism -- CPP directed oligonucleotide exon skipping in animal models of Duchenne muscular dystrophy -- PTD-DRBD siRNA delivery -- A non-covalent peptide-based strategy for siRNA delivery -- Application of PepFect peptides for the delivery of splice correcting oligonucleotides -- Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-Nucleoducin -- Enhanced cellular delivery of cell-penetrating peptide -peptide nucleic acid conjugates by photochemical internalization (PCI) -- Identification of homing peptides using the in vivo phage display technology -- Measuring the action of CPP-siRNA conjugates in the lung -- Intracellular delivery of nanoparticles with CPPs -- Multifunctional CPP polymer system for tumor-targeted pDNA and siRNA delivery -- Cell Penetrating Penta-Peptides (CPP5s) and Bax Inhibiting Peptides (BIPs): Protocol for their application -- PAIR technology: exon-specific RNA binding protein isolation in live cells -- Quantitation of cellular and topical uptake of luciferin oligoarginine conjugates -- Industrial-scale manufacturing of a possible oligonucleotide cargo CPP based drug -- Application of a fusiogenic peptide GALA for intracellular delivery -- Therapeutic Applications of Cell-Penetrating Peptides -- Nonclinical and clinical experiences with CPP-based self assembling peptide systems in topical drug development.
Keywords: Cell biology; Cell Biology