Bone Research Protocols - Helfrich, Miep H.; Ralston, Stuart H.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
Primary Human Osteoblast Cultures -- Osteoblast Isolation from Murine Calvaria and Long Bones -- Rat Osteoblast Cultures -- Isolation of primary avian Osteocytes -- Isolation of Mouse Osteocytes using Cell Fractionation for Gene Expression Analysis -- Studying Osteocyte Function using the Cell Lines MLO-Y4 and MLO-A5 -- Isolation, Differentiation and Characterisation of Human Bone Marrow Stem Cells in vitro and in vivo -- Rodent Osteoclast Cultures -- Isolation and Culture of Primary Chicken Osteoclasts -- ISOLATION AND PURIFICATION OF RABBIT OSTEOCLASTS -- Generation of Human Osteoclasts from Peripheral Blood -- Osteoclast Formation in Mouse Co-Cultures -- Rankl- Mediated Osteoclast Formation from Murine RAW 264.7 Cells -- Transfection of Osteoclasts and Osteoclast Precursors -- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation -- Analysis of Transcriptional Regulation in Bone Cells -- Extraction of Nucleic Acids from Bone -- Analysis of Gene Expression in Bone by Quantitative RT/PCR -- Histomorphometry in Rodents -- Studying Gene Expression in Bone by in situ Hybridization -- Immunostaining of Skeletal Tissue -- Techniques for the Study of Apoptosis in Bone -- Transmission Electron Microscopy of Bone -- Scanning Electron Microscopy of Bone -- Fluorescence Imaging of Osteoclasts using Confocal Microscopy -- Live Imaging of Bone Cell and Organ Cultures -- Analysis of Bone Architecture in Rodents using Micro Computed Tomography -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- Quantitative X-ray Imaging of Rodent Bone by Faxitron -- Bioluminescence Imaging of Bone Metastasis in Rodent -- Fourier Transform Infrared Imaging of Bone -- Raman Microscopy of Bone -- The Calvarial Injection Assay -- Ovariectomy/Orchidectomy in Rodents -- Mechanical Properties of Bone ex vivo -- Mechanical Stimulation of Bone Cells using Fluid Flow -- using CELL and organ culture models TO analyse responses OF bone cellS to mechanical STIMULATION .In vivo Mechanical Loading.
Keywords: Cell biology; Developmental biology; Animal anatomy; Cell Biology; Developmental Biology; Animal Anatomy / Morphology / Histology