Chromosomal Mutagenesis - Pruett-Miller, Shondra M.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Genome Editing by Targeted Chromosomal Mutagenesis -- piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells -- Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy -- Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA -- Genome Engineering Using Adeno-Associated Virus (AAV) -- Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization -- Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform -- Ligation-Independent Cloning (LIC) Assembly of TALENs -- Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications -- Genome Engineering Using CRISPR-Cas9 System -- Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases -- Endogenous Gene Tagging with Fluorescent Proteins -- Silencing Long Non-Coding RNAs with Genome-Editing Tools -- Gene Editing Using ssODNs with Engineered Endonucleases -- Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases -- Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells -- Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models -- Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection -- Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains.
Keywords: Human genetics; Genetic engineering; Human Genetics; Genetic Engineering