Chloroplast Research in Arabidopsis : Methods and Protocols, Volume II - Jarvis, R. Paul.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
One- and Two-Dimensional Blue Native (BN) PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes -- Analysis of Thylakoid Protein Complexes by Two Dimensional Electrophoretic Systems -- Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification -- Studying Interactions between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation (BiFC) and Förster Resonance Energy Transfer (FRET) -- Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods -- Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis -- Use of Transcriptomics to Analyse Chloroplast Processes in Arabidopsis -- Use of Non-Aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis -- Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis -- Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies -- Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis -- Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies -- Preparation and Proteomic Analysis of Chloroplast Ribosomes -- The Workflow for Quantitative Proteome Analysis of Chloroplast Development and Differentiation, Chloroplast Mutants, and Protein Interactions by Spectral Counting -- Use of Phosphoproteomics to Study Post-Translational Protein Modifications in Arabidopsis Chloroplasts -- Use of a Pulse-Amplitude Modulated (PAM) Chlorophyll Fluorometer to Study the Efficiency of Photosynthesis in Arabidopsis Plants -- Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves -- Measurement of the ΔpH and Electric Field Developed Across Arabidopsis Thylakoids in the Light -- Measurement of Chloroplast ATP Synthesis Activity in Arabidopsis -- Methods for Analysis of Photosynthetic Pigments and Steady-State Levels of Intermediates of Tetrapyrrole Biosynthesis -- Analysis of Starch Metabolism in Chloroplasts -- The Analysis of Lipid Content and Quality in Arabidopsis Plastids.
Keywords: Plant science; Botany; Plant biochemistry; Plant Sciences; Plant Biochemistry