Checkpoint Controls and Cancer : Volume 2: Activation and Regulation Protocols - Schönthal, Axel H.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Protocols for the Study of Checkpoint Regulatory Components -- Analysis of RB Action in DNA Damage Checkpoint Response -- Interaction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell Cycle -- Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Analysis -- Functional Analysis of CDK Inhibitor p21WAF1 -- Analysis of p21CDKN1A Recruitment to DNA Excision Repair Foci in the UV-Induced DNA Damage Response -- Quantitative Determination of p16 Gene Expression by RT-PCR -- Measuring Cyclin-Dependent Kinase Activity -- Determination of the Catalytic Activities of mTOR and Other Members of the Phosphoinositide-3-Kinase-Related Kinase Family -- CHK1 Kinase Activity Assay -- Assaying Cdc25 Phosphatase Activity -- Analyzing the Regulation and Function of ATM -- Use of siRNA to Study the Function of MDC1 in DNA Damage Responses -- Functional Analysis of APC-Cdh1 -- Purification of the Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa Cells -- Analysis of the Spindle-Assembly Checkpoint in HeLa Cells -- Functional Analysis of the Spindle-Checkpoint Proteins Using an In Vitro Ubiquitination Assay -- Studying Consequences of Checkpoint Pathway Activation -- Analysis of Checkpoint Responses to Histone Deacetylase Inhibitors -- Biochemical Analysis of the Cell Cycle and Cell Cycle Checkpoints in Transiently Transfected Cells After Collection With Magnetic Beads -- Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates -- Analyzing Cell Cycle Checkpoints After Ionizing Radiation -- FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells -- Analysis of Cell Cycle by Flow Cytometry -- Analyzing Markers of Apoptosis In Vitro -- Analysis of Telomerase Activity and Telomere Function in Cancer.
Keywords: Cancer research; Cancer Research