Chloroplast Research in Arabidopsis : Methods and Protocols, Volume I - Jarvis, R. Paul.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis -- Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy -- Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins -- Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts -- Visualisation of Stromules on Arabidopsis Plastids -- Analysis of Chloroplast Movement and Relocation in Arabidopsis -- Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots -- Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy -- Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method -- Isolation, Quantification, and Analysis of Chloroplast DNA -- Measurement of Transcription Rates in Arabidopsis Chloroplasts -- Studying the Structure and Processing of Chloroplast Transcripts -- In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts -- Studying Translation in Arabidopsis Chloroplasts -- Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts -- In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins -- Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays -- Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions -- Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays -- Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction -- Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases.
Keywords: Plant science; Botany; Plant biochemistry; Plant Sciences; Plant Biochemistry