Cancer Cell Culture : Methods and Protocols - Langdon, Simon P.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
to Cancer Cell Culture -- Basic Principles of Cancer Cell Culture -- Essential Techniques of Cancer Cell Culture -- Characterization and Authentication -- Characterization and Authentication of Cancer Cell Lines -- Authentication of Cancer Cell Lines by DNA Fingerprinting -- Cytogenetic Characterization of Tumor Cell Lines -- Isolation and Culture of Specialized Cell Types -- Isolation and Culture of Colon Cancer Cell Lines -- Isolation and Culture of Melanoma Cell Lines -- Isolation and Culture of Human Brain Tumor Cells -- Isolation and Culture of Renal Cancer Cell Lines -- Isolation and Culture of Prostate Cancer Cell Lines -- Isolation and Culture of Ovarian Cancer Cell Lines -- Isolation and Culture of Leukemia Cell Lines -- Functional Assays in Cell Culture -- Cell Sensitivity Assays: Clonogenic Assay -- Cell Sensitivity Assays: The MTT Assay -- PARP Cleavage as a Means of Assessing Apoptosis -- Detection of Apoptosis by the TUNEL Assay -- Apoptosis Measurement by Annexin V Staining -- Cell Adhesion Assays -- Radial Monolayer Cell Migration Assay -- Invasion and Motility Assays -- Identification of Senescence in Cancer Cells -- Angiogenesis Assays -- Flow Cytometric DNA Analysis of Human Cancer Cell Lines -- Modification of Cancer Cells -- DNA-Mediated Gene Transfer -- Development of Drug-Resistant Models -- Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene -- The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts -- Coculture Systems -- Coculture of Prostate Cancer Cells -- Coculture of Ovarian Cells Using Porous Tissue Culture Inserts -- Cell Line Contamination -- Cell Culture Contamination -- Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction -- Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics.
Keywords: Oncology .; Oncology