Chaperonin Protocols - Schneider, Christine; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2000)
Purification of Archaeal Chaperonin from Sulfolobus shibatae -- Purification of Hsp60 from Thermus thermophilus -- Purification of GroEL from an Overproducing E. coliStrain -- Purification of GroES from an Overproducing E. coliStrain -- Purification of the Gp31 Co-chaperonin of BacteriophageT4 -- Removing Trace Fluorescent Contaminants from GroEL Preparations -- Assembly and Disassembly of GroEL and GroES Complexes -- GroEL/GroES Interaction Assayed by Protease Protection -- Determination of Chaperonin Activity In Vivo -- Interaction of Nonnative Polypeptide Substrates with the Escherichia coli Chaperonin GroEL -- Prevention of Rhodanese Aggregation by the Chaperonin GroEL -- Refolding of Bovine Mitochondrial Rhodanese by Chaperonins GroEL and GroES -- Assay of Malate Dehydrogenase -- Assay of Chaperonin-Assisted Refolding of Citrate Synthase -- Purification of Yeast Mitochondrial Hsp60 -- Preparation of Recombinant Human Hsp10 -- Purification of the Cytosolic ChaperoninTRiC from Bovine Testis -- Monitoring Actin Folding -- Folding Assays -- Purification of Prefoldin -- Purification of GimC from Saccharomyces cerevisiae -- Analysis of Eukaryotic Molecular Chaperone Complexes Involved in Actin Folding.
Keywords: Biochemistry; Biochemistry, general