Cancer Cell Culture : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edición: | 2nd ed. 2011. |
| Colección: | Methods in Molecular Biology,
731 |
| Materias: |
Tabla de Contenidos:
- Cancer Biology
- Principles of Cancer Cell Culture
- Storage of Cell Lines
- Characterization and Authentication of Cancer Cell Lines: An Overview
- Online Verification of Human Cell Line Identity by STR DNA Typing
- Cytogenetic Analysis of Cancer Cell Lines
- Cell Culture Contamination
- Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction
- Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics
- Quality Assurance and Good Laboratory Practice
- Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning.- Isolation and Culture of Colon Cancer Cells and Cell Lines
- Isolation and Culture of Melanoma and Naevus Cells and Cell Lines
- Isolation and Culture of Squamous Cell Carcinomalines
- Isolation and Culture of Ovarian Cancer Cells and Cell Lines
- Establishment and Culture of Leukemia-Lymphoma Cell Lines
- Isolation of Inflammatory Cells from Human Tumors
- Isolation of Endothelial Cells from Human Tumors
- Cellular Chemosensitivity Assays: An Overview
- Cell Sensitivity Assays: The MTT Assay
- Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay
- The Differential Staining Cytotoxicity (DiSC) Assay: A Review
- Real-time Cytotoxicity Assays
- FPurification of Annexin V and its use in the Detection of Apoptotic Cells
- Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay
- Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR
- Cell Migration and Invasion Assays
- Angiogenesis Assays
- Flow Cytometric DNA Analysis of Human Cancers and Cell Lines
- Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines
- Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transciptase Polymerase Chain Reaction.- Proteomic Evaluation of Cancer Cells - Identification of Cell Surface Proteins
- Development of Rituximab-resistant B-NHL Clones: An In Vitro Model for Studying Tumor Resistance to Monoclonal Antibody-mediated Immunotherapy.- High Throughput Screening of Natural Products for Cancer Therapy: A Review
- Analysis of Drug Interactions.- Transfection and DNA-mediated Gene Transfer
- Drug Design and Testing: Profiling of Anti-proliferative Agents for Cancer Therapy Using a Cell-based Methyl-[3H]-Thymidine Incorporation Assay
- Feeder Layers: Co-culture with Non-neoplastic Cells
- Xenotransplantation of Breast Cancers.