Bacterial Chemosensing : Methods and Protocols - Manson, Michael D.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
The Diversity of Bacterial Chemosensing -- Transmembrane Signal Transduction in Bacterial Chemosensing -- Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay -- Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries -- A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-Linear Gradients -- Visualizing Chemoattraction of Planktonic Cells to a Biofilm -- Labeling Bacterial Flagella with Fluorescent Dyes -- All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins -- Mutational Analysis of Binding Protein/Chemoreceptor Interactions -- In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway -- FRET Analysis of the Chemotaxis Pathway Response -- Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout -- Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation -- Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid-Aqueous Interface -- Analyzing Chemoreceptor Interactions In Vivo with the Tri-Functional Crosslinker TMEA -- Use of Cryo-EM to Study the Structure of Chemoreceptor Arrays In Vivo -- Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis -- Bacterial Chemoreceptor Imaging at High Spatio-Temporal Resolution Using Photoconvertible Fluorescent Proteins -- Imaging of Single Dye-Labeled Chemotaxis Proteins in Live Bacteria Using Electroporation -- Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing -- Chemotaxis to Atypical Chemoattractants by Soil Bacteria -- Screening Chemoreceptor-Ligand Interactions by High-Throughput Thermal-Shift Assays -- High-Throughput Screening to Identify Chemoreceptor Ligands.- Identification of Specific Ligands for Sensory Receptors by Small-Molecule Ligand Arrays and Surface Plasmon Resonance.- Fluorescence Measurement of Kinetics of CheY Autophosphorylation with Small Molecule Phosphodonors -- Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY -- Quantitative Modeling of Flagellar Motor-Mediated Adaptation -- Molecular Modeling of Chemoreceptor:Ligand Interactions -- Phylogenetic and Protein Sequence Analysis of Bacterial Chemoreceptors.
Keywords: Microbiology; Microbiology