Fungal Secondary Metabolism : Methods and Protocols - Keller, Nancy P.; Turner, Geoffrey; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
Library Preparation and Data Analysis Packages for Rapid Genome Sequencing -- Bioinformatics Approaches and Software for Detection of Secondary Metabolic Gene Clusters -- Media and Growth Conditions for Induction of Secondary Metabolite Production -- Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays -- The "FERMEX" Method for Metabolite-Enriched Fungal Extracts -- The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites -- Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri -- Production, Detection, and Purification of Clavine-Type Ergot Alkaloids -- Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS) -- Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans -- Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis -- Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host -- Multi-Copy Genetic Screen in Aspergillus nidulans -- Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification -- Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra -- Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi -- Targeted Proteomics for Metabolic Pathway Optimization -- Investigation of In Vivo Protein Interactions in Aspergillus Spores -- Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus -- Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi -- Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects.
Keywords: Microbiology; Gene expression; Microbiology; Eukaryotic Microbiology; Gene Expression