Cyclooxygenases : Methods and Protocols - Ayoub, Samir S.; Flower, Roderick J.; Seed, Michael; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
In Vitro Protocols for Studying Expression and Activity of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns -- In Vitro Cyclo-oxygenase Expression and Activity Protocols: Introduction to Part I -- Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites -- Purification of Recombinant Human COX-1 and COX-2 -- Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2 -- Expression of Cyclooxygenase Isoforms in the Baculovirus Expression System -- Peroxidase Active Site Activity Assay -- Study of Inhibitors of the PGH Synthases for which Potency Is Regulated by the Redox State of the Enzymes -- Different Methods for Testing Potential Cyclooxygenase-1 and Cyclooxygenase-2 Inhibitors -- In Vitro Cyclooxygenase Activity Assay in Tissue Homogenates -- Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site -- In Vitro Assays for Cyclooxygenase Activity and Inhibitor Characterization -- Extraction and Measurement of Prostanoids and Isoprostanes -- Extraction and Measurement of Prostanoids and Isoprostanes: Introduction to Part II -- Prostanoid Extraction and Measurement -- Measurement of 8-Iso-Prostaglandin F2? in Biological Fluids as a Measure of Lipid Peroxidation -- In Vivo Models to Study Involvement of Cyclooxygenase Products in Health and Disease -- In Vivo Models to Study Cyclooxygenase Products in Health and Disease: Introduction to Part III -- Protocols to Assess the Gastrointestinal Side Effects Resulting from Inhibition of Cyclo-Oxygenase Isoforms -- Cyclooxygenase Enzymes and Their Products in the Carrageenan-Induced Pleurisy in Rats -- Iloprost-Induced Nociception: Determination of the Site of Anti-nociceptive Action of Cyclooxygenase Inhibitors and the Involvement of Cyclooxygenase Products in Central Mechanisms of Nociception.
Keywords: Pharmacology; Biochemical engineering; Pharmacology/Toxicology.; Biochemical Engineering