Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing - Aransay, Ana M.; Lavín Trueba, José Luis.; SpringerLink (Online service) (Cham : Springer International Publishing : Imprint: Springer, 2016)
The High Throughput Sequencing technologies triple-W discussion: Why to use HTS, What is the optimal HTS method to use, Which data analysis workflow to follow -- Whole genome sequencing recommendations -- Counseling for targeted DNA region re-sequencing -- Transcriptome profiling strategies -- Differential mRNA Alternative Splicing -- microRNA discovery and expression analysis in animals -- Analysis of Long Non-coding RNAs in RNA-seq data -- Ribosome profiling -- Genome-wide analysis of DNA methylation patterns by high-throughput sequencing -- Characterization of DNA-protein interactions: Design and analysis of ChIP-seq experiments -- PAR-CLIP: A genomic technique to dissect RNA-protein interactions -- Metagenomic design and sequencing -- A hitchhiker's guide to Metatranscriptomics -- Eukaryotic Single-Cell mRNA Sequencing -- Eukaryotic Single-Cell DNA Sequencing -- Submitting data to a public repository, the final step of a successful HTS experiment.
Keywords: Human genetics; Systems biology; Biostatistics; Human Genetics; Systems Biology; Biostatistics