Fluorescence Spectroscopy and Microscopy : Methods and Protocols - Engelborghs, Yves.; Visser, Antonie J.W.G.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
Part I: Steady-State Fluorescence Spectroscopy (SSFS). How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra -- Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions -- Quantitative Fluorescence Spectral Analysis of Protein Denaturation -- High-Pressure Fluorescence Applications -- Part II: Time-Resolved Fluorescence Spectroscopy (TRFS). Frequency Domain Fluorometry: Theory and Application -- Polar Plot Representation of Time-Resolved Fluorescence -- Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics -- MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes -- Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins -- Global Analysis of Time-Resolved Fluorescence Data -- Nanometrology -- Upconversion Spectrophotofluorometry -- Subpicosecond Kerr-Gate Spectrofluorometry -- Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay -- Part III: Fluorescent Probe Development (FPD). Biosynthetic Incorporation of Trp Analogs in Proteins -- Optimization of Fluorescent Proteins -- Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family -- Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP). Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope -- Part V: Fluorescence Microscopy: Förster Resonance Energy Transfer Imaging (FRETim). A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging -- Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM). Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors -- Global Analysis of FRET- FLIM Data in Live Plant Cells -- Time-Resolved Fluorescence Anisotropy Imaging -- Multi-Modal Fluorescence Imaging Spectroscopy -- Part VII: Fluorescence Microscopy: Fluorescence Fluctuation Spectroscopy (FFS). Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells -- Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells -- Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS) -- PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching -- Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes -- Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane -- Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS -- Quantitative Study of Protein-Protein Interactions in Live Cell by Dual Color Fluorescence Lifetime Cross-Correlation Spectroscopy -- Brightness Experiments -- Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy -- Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy -- Part VIII: Fluorescence Microscopy: Single-Molecule Fluorescence Spectroscopy (smFS). Single-Molecule Fluorescence of Nucleic Acids -- Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Above the Diffraction Limit of Light.
Keywords: Radiology; Microscopy; Spectroscopy; Imaging / Radiology; Biological Microscopy; Spectroscopy/Spectrometry.