Glyco-Engineering : Methods and Protocols - Castilho, Alexandra; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Current Approaches to Engineering N-Linked Protein Glycosylation in Bacteria -- Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli -- GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes -- Production of Glycoproteins with Asparagine-Linked N-Acetylglucosamine in Escherichia coli -- Glyco-Engineering O-Antigene-Based Vaccines and Diagnostics in E. coli -- Progress in Yeast Glycosylation Engineering -- Protein Production with a Pichia pastoris OCH1 Knock-Out Strain in Fed-Batch Mode -- Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology -- Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Production of Therapeutic Glycoproteins -- An Overview and History of Glyco-Engineering in Insect Expression Systems -- SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylation -- Engineering Sialic Acid Synthesis Ability in Insect Cells -- Engineering N-Glycosylation Pathway in Insect Cells: Suppression of β-N-Acetylglucosaminidase and Expression of β-1,4-Galactosyltransferase -- N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements -- Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typical Glycosylation in Moss Bioreactors -- Im"plant"ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation -- Transient Glyco-Engineering of N. benthamiana Aiming at the Synthesis of Multi-Antennary Sialylated Proteins -- Subcellular Targeting of Proteins Involved in Modification of plant N- and O-Glycosylation -- Assembly of Multigene Constructs Using Golden Gate Cloning -- Strategies for Engineering Protein N-Glycosylation Pathway in Mammalian Cells -- Glycan Remodelling with Processing Inhibitors and Lectin-Resistant Eukaryotic Cells -- Production of Highly Sialylated Recombinant Glycoproteins Using Ricinus communis Agglutinin-I-Resistant CHO Glycosylation Mutants -- Metabolic Glyco-Engineering in Eukaryotic Cells and Selected Applications -- Evaluation of Quenching and Extraction Methods for Nucleotide/Nucleotide Sugar Analysis -- Chemoenzymatic Glyco-Engineering of Monoclonal Antibodies -- Chemical Polysialylation of Recombinant Human Proteins -- Site Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS) -- Mass Spectrometric Analysis of Oligo- and Polysialic Acids -- Isomer-Specific Analysis of Released N-Glycans by LC-ESI MS/MS with Porous Graphitized Carbon.
Keywords: Proteins .; Protein Science