E. coli Gene Expression Protocols - Vaillancourt, Peter E.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
Cold-Inducible Promoters for Heterologous Protein Expression -- Dual-Expression Vectors for Efficient Protein Expression in Both E. coli and Mammalian Cells -- A Dual-Expression Vector Allowing Expression in E. coli and P. pastoris, Including New Modifications -- Purification of Recombinant Proteins from E. coli by Engineered Inteins -- Calmodulin as an Affinity Purification Tag -- Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification -- Maltose-Binding Protein as a Solubility Enhancer -- Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli -- Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli -- Assessment of Protein Folding/Solubility in Live Cells -- Improving Heterologous Protein Folding via Molecular Chaperone and Foldase Co-Expression -- High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins -- Co-Expression of Proteins in E. coli Using Dual Expression Vectors -- Small-Molecule Affinity-Based Matrices for Rapid Protein Purification -- Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli -- Screening Peptide/Protein Libraries Fused to the ? Repressor DNA-Binding Domain in E. coli Cells -- Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System -- Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions -- Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli -- Hyperphage -- Combinatorial Biosynthesis of Novel Carotenoids in E. coli -- Using Transcriptional-Based Systems for In Vivo Enzyme Screening -- Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis.
Keywords: Biochemistry; Cell biology; Biochemistry, general; Cell Biology