Directed Evolution Library Creation : Methods and Protocols - Arnold, Frances H.; Georgiou, George; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
Mutagenesis and Recombination Methods -- Generating Mutant Libraries Using Error-Prone PCR -- Preparing Libraries in Escherichia coli -- Preparing Libraries in Saccharomyces cerevisiae -- Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP) -- Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis -- Production of Randomly Mutated Plasmid Libraries Using Mutator Strains -- Evolution of Microorganisms Using Mutator Plasmids -- Random Insertion and Deletion Mutagenesis -- Random Oligonucleotide Mutagenesis -- Saturation Mutagenesis -- DNA Shuffling -- Family Shuffling with Single-Stranded DNA -- In Vitro DNA Recombination by Random Priming -- Staggered Extension Process (StEP) In Vitro Recombination -- RACHITT -- The Creation of ITCHY Hybrid Protein Libraries -- Preparation of SCRATCHY Hybrid Protein Libraries -- Sequence Homology-Independent Protein Recombination (SHIPREC) -- Producing Chimeric Genes by CLERY -- Analysis of Library Diversity -- Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization -- Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays -- Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays.
Keywords: Cell biology; Cell Biology