ERK Signaling : Methods and Protocols - Jimenez, Gerardo; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
How Genetics has Helped Piece Together the MAPK Signaling Pathway -- In Vitro Enzyme Kinetics Analysis of EGFR -- High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells -- Structural Studies of ERK2 Protein Complexes -- Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk -- Assaying Activation and Subcellular Localization of ERK in Cells and Tissues -- Detection and Functional Analysis of SUMO-Modified MEK -- Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag -- High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates -- Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE -- Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation -- Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions -- The Nuclear Translocation of ERK -- Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA) -- Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors -- Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers -- Co-Culture Activation of MAP Kinase in Drosophila S2 Cells -- Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling -- 3D Organotypic Culture Model to Study Components of ERK Signaling -- Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway -- Genome-Wide Analysis of RAS/ERK Signaling Targets -- Probing Chromatin Modifications in Response to ERK Signaling -- Analyzing pERK Activation during Planarian Regeneration -- Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development -- Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images -- Using CRISPR-Cas9 to Study ERK Signaling in Drosophila -- Analyzing ERK Signal Dynamics during Zebrafish Somitogenesis -- Modeling RASopathies with Genetically Modified Mouse Models -- Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway.
Keywords: Biochemistry; Biochemistry, general