Cytokine Protocols - De Ley, Marc.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
Large-Scale Generation of Plasmids that Express Type I Interferon -- Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay -- Modulation of the Interferon-? Signal by Transfection of Cells with an Antisense-RNA Expressing Vector -- Competitive RT-PCR to Quantify Small Amounts of mRNA -- In SituHybridization for Cytokines in Human Tissue Biopsies -- Purification, Identification, and Synthesis of Chemokines -- Receptor Isolation and Characterization -- Crystallization of Cytokine-Receptor Complexes -- Biosensor Analysis of Receptor-Ligand Interactions -- Analysis of SH2 Ligands and Identification of Sites of Interaction -- Assays for Antiviral Activity -- Assays for Cytotoxicity -- Assays for Chemotaxis -- In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor -- Development of a Mammalian Tet-On Expression Cell Line -- Immunohistochemical Detection of Cytokines in Human Tissue Sections -- Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level -- Detection of Cytokine Signal Transduction "Cross-Talk" in Leukocyte Activation -- Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production.
Keywords: Immunology; Immunology