Current Laboratory Methods in Neuroscience Research - Xiong, Huangui; Gendelman, Howard E.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Springer, 2014)
Section 1: Neurohistological techniques -- 1. Brain tissue preparation, sectioning and staining -- 2. Brain stereotactic injections -- 3. Tract tracing at light and electron microscopy -- 4. Stereological analysis of neurological tissues -- Section 2: In vitro preparations -- 5. Preparation and use of rodent hippocampal brain slices -- 6.  Single-Cell Neuronal Dissociation For Electrophysiological Studies -- 7. Isolation and Culture of Human Neurons, Microglia and Astrocytes -- 8. Isolation and Culture of Neural Stem/progenitor Cells -- 9. Isolation of mitochondria from brain tissue and cells -- 10. Isolation of synaptosomes from archived brain tissues -- 11. Xenopus oocytes isolation and microinjection -- Section 3: Leukocyte isolation and application in neuroscience -- 12. Centrifugal elutriation for studies of neuroimmunity -- 13. Flow cytometry in neuroscience research -- Section 4: Standard Laboratory Nucleic Acid and Protein Detections -- 14. Western Blotting Technique in Biomedical Research -- 15. Polymerase chain reaction (PCR) and Real-time RT PCR -- Section 5: Nanomedicine -- 16. Nanoformulations -- 17. Neuronanomedicine -- Section 6: Bioimaging -- 18. Confocal imaging of nerve cells -- 19. Magnetic resonance imaging (MRI) -- 20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging -- 21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography -- 22. Multiphoton Brain Imaging -- Section 7: Neuroelectrophysiology -- 23. Techniques for Extracellular Recordings -- 24. "Blind" patch-clamp recordings from rodent hippocampal slices -- 25. Whole-cell patch clamp recordings -- Section 8: Immunohistochemistry and autoradiography -- 26. Analysis of Receptor Binding And Quantitative Autoradiography -- 27. Techniques in Immunohistochemistry and immunocytochemistry -- Section 9: Analysis of Gene Expression -- 28. Protein identification by mass spectrometry: Proteomics -- 29. DNA Microarrays as a Tool for Neurosciences Research -- 30. Metabolomics -- 31. Bioinformatic Methods and Resources for Neuroscience Research -- Section 10: Animal Models: Behavior and Pathology -- 32. Motor function in rodent models of neurodegenerative disorders -- 33. Humanized mice -- 34. Animal models for PD and ALS -- 35. Animal models for Alzheimer's Disease -- 36. Animal Models: Behavior and Pathology.
Keywords: Neurosciences; Neurology .; Neurobiology; Neurosciences; Neurology; Neurobiology