Drug Safety Evaluation : Methods and Protocols - Gautier, Jean-Charles.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Developing Combination Drugs in Preclinical Studies -- Preclinical Evaluation of Juvenile Toxicity -- Necropsy and Sampling Procedures in Rodents -- Histopathology Procedures: From Tissue Sampling to Histopathological Evaluation -- Principles and Methods of Immunohistochemistry -- Tissue Microarrays and Digital Image Analysis -- Micronucleus Assay and Labeling of Centromeres with FISH Technique -- The Use of Bacterial Repair Endonucleases in the Comet Assay -- Manual Whole-Cell Patch-Clamping of the HERG Cardiac K+ Channel -- Generation and Analysis of Transcriptomics Data -- Protocols of Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) to Investigate Mechanisms of Toxicity -- Protocols and Applications of Cellular Metabolomics in Safety Studies Using Precision-Cut Tissue Slices and Carbon 13 NMR -- Statistical Analysis of Quantitative RT-PCR Results -- Evaluation of Mitochondrial Respiration in Cultured Rat Hepatocytes -- FETAX Assay for Evaluation of Developmental Toxicity -- Evaluation of Embryotoxicity Using the Zebrafish Model -- Protocols of In vitro Protein Covalent Binding Studies in Liver -- Utilization of MALDI-TOF to Determine Chemical-Protein Adduct Formation In vitro -- Utilization of LC-MS/MS Analyses to Identify Site-Specific Chemical Protein Adducts In vitro -- 1-Dimensional Western Blotting Coupled to LC-MS/MS Analysis to Identify Chemical-Adducted Proteins in Rat Urine -- Identification of Chemical Adducted Proteins in Urine by Multi-Dimensional Protein Identification Technology (LC/LC-MS/MS) -- Optimization of SELDI for Biomarker Detection in Plasma -- Differential Proteomics Incorporating iTRAQ Labelling and Multi-Dimensional Separations -- NMR and MS Methods for Metabonomics -- Absolute Quantification of Toxicological Biomarkers by Multiple Reaction Monitoring.
Keywords: Toxicology; Medical laboratories; Pharmacology/Toxicology.; Laboratory Medicine