Fluorescent Energy Transfer Nucleic Acid Probes : Designs and Protocols - Didenko, Vladimir V.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2006)
Design of Energy Transfer Probes -- Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes -- Choosing Reporter-Quencher Pairs for Efficient Quenching Through Formation of Intramolecular Dimers -- Energy Transfer Probes for DNA and RNA Hybridization Detection and Monitoring -- Detection of DNA Hybridization Using Induced Fluorescence Resonance Energy Transfer -- Detecting RNA/DNA Hybridization Using Double-Labeled Donor Probes With Enhanced Fluorescence Resonance Energy Transfer Signals -- Energy Transfer Probes for DNA Breaks Detection and DNA Cleavage Monitoring -- Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections -- Using Molecular Beacons for Sensitive Fluorescence Assays of the Enzymatic Cleavage of Nucleic Acids -- A Continuous Assay for DNA Cleavage Using Molecular Break Lights -- Monitoring of DNA Synthesis and Amplification Using Energy Transfer Probes -- Homogenous Detection of Nucleic Acids Using Self-Quenched Polymerase Chain Reaction Primers Labeled With a Single Fluorophore (LUX™ Primers) -- Use of Self-Quenched, Fluorogenic LUX™ Primers for Gene Expression Profiling -- TaqMan® Reverse Transcriptase-Polymerase Chain Reaction Coupled With Capillary Electrophoresis for Quantification and Identification of bcr-abl Transcript Type -- Quantitative TaqMan® Assay for the Detection and Monitoring of Cytomegalovirus Infection in Organ Transplant Patients -- Real-Time Detection and Quantification of Telomerase Activity Utilizing Energy Transfer Primers -- DNA Sequence Analysis and Mutation Detection Using Fluorescence Energy Transfer -- Invader® Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation -- Real-Time Quantitative Polymerase Chain Reaction Analysis of Mitochondrial DNA Point Mutation -- Multiplex Single-Nucleotide Polymorphism Detection by Combinatorial Fluorescence Energy Transfer Tags and Molecular Affinity -- High-Throughput Genotyping With Energy Transfer-Labeled Primers -- Determination of Distance and DNA Folding -- Distance Determination in Protein-DNA Complexes Using Fluorescence Resonance Energy Transfer -- Multi-Fluorophore Fluorescence Resonance Energy Transfer for Probing Nucleic Acids Structure and Folding -- Dna-Based Biosensors Utilizing Energy Transfer -- Fluorescent DNAzyme Biosensors for Metal Ions Based on Catalytic Molecular Beacons -- Fluorescent Energy Transfer Readout of an Aptazyme-Based Biosensor -- Fluorescence Resonance Energy Transfer in the Studies of Guanine Quadruplexes -- Solution-Phase Molecular-Scale Computation With Deoxyribozyme-Based Logic Gates and Fluorescent Readouts.
Keywords: Biochemistry; Cell biology; Biochemistry, general; Cell Biology