Electron Crystallography of Soluble and Membrane Proteins : Methods and Protocols - Schmidt-Krey, Ingeborg; Cheng, Yifan; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
Introduction to Electron Crystallography -- Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis -- Two-Dimensional Crystallization of Membrane Proteins by Reconstitution through Dialysis -- Monolayer Two-Dimensional Crystallization -- Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples -- Low Dose Techniques and Cryo-Electron Microscopy -- Grid Preparation for Cryo-Electron Microscopy -- Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins -- The Collection of High-Resolution Electron Diffraction Data -- Image Processing of 2D Crystal Images -- Merging of Image Data in Electron Crystallography -- Evaluation of Electron Crystallographic Data From Images of Two-Dimensional Crystals -- Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy -- Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement -- High-Throughput Methods for Electron Crystallography -- Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening -- Automation of Data Acquisition in Electron Crystallography -- Automation of Image Processing in Electron Crystallography -- Choice and Maintenance of Equipment for Electron Crystallography -- Future Developments in Instrumentation for Electron Crystallography -- Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction -- Single Particle Electron Microscopy -- Electron Tomography of Paracrystalline 2D Arrays -- High-Resolution Imaging of 2D Outer Membrane Protein F (OmpF) Crystals by Atomic Force Microscopy -- Determination of Soluble and Membrane Protein Structures by X-Ray Crystallography -- Solution Nuclear Magnetic Resonance (NMR) Spectroscopy -- Structure-Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography -- Lipid Monolayer and Sparse Matrix Screening for Growing Two-Dimensional Crystals for Electron Crystallography: Methods and Examples -- Processing of Electron Diffraction Patterns with the XDP Program -- Future Directions of Electron Crystallography.
Keywords: Proteins .; Microscopy; Protein Science; Biological Microscopy