DNA Topoisomerase Protocols : Volume II: Enzymology and Drugs - Osheroff, Neil.; Bjornsti, Mary-Ann.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Assaying DNA Topoisomerase I Relaxation Activity -- DNA Topoisomerase II-Catalyzed DNA Decatenation -- Plasmid DNA Supercoiling by DNA Gyrase -- Analyzing Reverse Gyrase Activity -- Topoisomerase II-Catalyzed ATP Hydrolysis as Monitored by Thin-Layer Chromatography -- Use of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II -- Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift Assay -- Filter Binding Assays for Topoisomerase-DNA Complexes -- DNATopoisomerase I-Mediated Nicking of Circular Duplex DNA -- Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives -- Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation -- Synthesis and Use of DNA Containing a 5?-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA Topoisomerases -- Isolation of Covalent Enzyme-DNA Complexes -- ICE Bioassay -- DNA-Unwinding Test Using Eukaryotic DNA Topoisomerase I -- Drug-DNA Interactions -- Quinolone Interactions with DNA and DNA Gyrase -- Bactericidal Assays for Fluoroquinolones -- Drug Toxicity in E. coli Cells Expressing Human Topoisomerase I -- Drug-Induced Cytotoxicity in Tissue Culture -- Analysis of Drug Uptake-Flow Cytometry -- Cell-Cycle Analysis of Drug-Treated Cells -- Assaying Drug-Induced Apoptosis -- Single-Strand Conformational Polymorphism Analysis of DNA Topoisomerases I and II -- PCR-Based Cloning of DNA Topoisomerase Genes -- Topoisomerase II-Catalyzed Relaxation and Catenation of Plasmid DNA -- Topoisomerase II-Mediated Cleavage of Plasmid DNA -- Drug-induced Stabilization of Covalent DNA Topoisomerase I-DNA Intermediates -- Studying DNATopoisomerase I-Targeted Drugs In the Yeast -- Yeast Systems for Demonstrating the Targets of Anti-Topoisomerase II Agents.
Keywords: Pharmacy; Pharmacy