GTPase Protocols : The Ras Superfamily - Manser, Ed.; Leung, Thomas; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2002)
General Protocols -- The GTPase Cycle How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases -- Preparation of GTPases for Structural and Biophysical Analysis -- Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes -- Fluorescence Methods in the Study of Small GTP-Binding Proteins -- Specific Methods -- Ras and Rac as Activators of Reactive Oxygen Species (ROS) -- Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs -- A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays -- Determination of the Activity of Rho-Like GTPases in Cells -- of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases -- Effects of Rho Family GTPases on Cell-Cell Adhesion -- Cell Motility and Invasion Assays -- Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases -- Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl Transferase -- Preparation of Myristoylated Arf1 and Arf6 Proteins -- ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins -- Arf6 and its Role in Cytoskeletal Modulation -- ARF GTPase-Activating Protein 1 -- The Use of Permeabilized Cell Systems to Study Nuclear Transport -- Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes -- Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton.
Keywords: Biochemistry; Biochemistry, general