E. coli Plasmid Vectors : Methods and Applications - Casali, Nicola; Preston, Andrew; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
The Function and Organization of Plasmids -- Choosing a Cloning Vector -- Escherichia coli Host Strains -- Chemical Transformation of E. coli -- Electroporation of E. coli -- DNA Transfer by Bacterial Conjugation -- Cosmid Packaging and Infection of E. coli -- Isolation of Plasmids from E. coli by Alkaline Lysis -- Isolation of Plasmids from E. coli by Boiling Lysis -- High-Purity Plasmid Isolation Using Silica Oxide -- High-Throughput Plasmid Extraction Using Microtiter Plates -- Isolation of Cosmid and BAC DNA from E. coli -- Preparation of Single-Stranded DNA from Phagemid Vectors -- Using Desktop Cloning Software to Plan, Track, and Evaluate Cloning Projects -- Cloning in Plasmid Vectors -- Extraction of DNA from Agarose Gels -- Cloning PCR Products with T-Vectors -- Construction of Genomic Libraries in ?-Vectors -- Rapid Screening of Recombinant Plasmids -- Restriction Analysis of Recombinant Plasmids -- Screening Recombinant DNA Libraries -- Sequencing Using Fluorescent-Labeled Nucleotides -- Site-Directed Mutagenesis Using the Megaprimer Method -- Site-Directed Mutagenesis by Inverse PCR -- Creating Nested DNA Deletions Using Exonuclease III -- Transposon and Transposome Mutagenesis of Plasmids, Cosmids, and BACs -- In Vitro Transcription and Translation -- Vectors for the Expression of Recombinant Proteins in E. coli -- Expression of Recombinant Proteins From lac Promoters -- Plasmid-Based Reporter Genes -- Plasmid-Based Reporter Genes.
Keywords: Biochemistry; Cell biology; Biochemistry, general; Cell Biology