Generation of cDNA Libraries : Methods and Protocols - Ying, Shao-Yao.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
Complementary DNA Libraries -- Rapid Amplification of cDNA Ends -- cDNA Generation on Paramagnetic Beads -- Construction of a Normalized cDNA Library by mRN-cDNA Hybridization and Subtraction -- Amplification of cDNA Ends Using PCR Suppression Effect and Step-Out PCR -- Use of Inverse PCR to Clone cDNA Ends -- Construction of Size-Fractionated cDNA Library Assisted by an In Vitro Recombination Reaction -- Construction of a Full-Length Enriched and a 5?-End Enriched cDNA Library Using the Oligo-Capping Method -- cDNA Library Construction Using In Vitro Transcriptional Amplification -- Amplification of Representative cDNA Pools from Microscopic Amounts of Animal Tissue -- Single-Cell cDNA Library Construction Using Cycling aRNA Amplification -- mRNA/cDNA Library Construction Using RNA-Polymerase Cycling Reaction -- Quality Assessment of cDNA Libraries -- Assessment of the Quality of mRNA Libraries by Agarose Gel Electrophoresis -- PACS RT-PCR -- Single-Cell mRNA Library Analysis by Northern Blot Hybridization -- Generation of cDNA Libraries for Profiling Gene Expression of Given Tissues or Cells -- Screening Poly [dA/dT(?)] cDNA for Gene Identification -- Generation of Longer cDNA Fragments from SAGE Tags for Gene Identification -- Generation of Full-Length cDNA Libraries Enriched for Differentially Expressed Genes -- Subtractive Hybridization for the Identification of Differentially Expressed Genes Using Uraci-DNA Glycosylase and Mung-Bean Nuclease -- Subtractive Cloning of Differential Genes Using RNA-PCR -- Strategy for Construction of a cDNA Encoding a Repetitive Amino Acid Sequence -- Preparing Lambda Libraries for Expression of Proteins in Prokaryotes or Eukaryotes -- Peptide Library Construction from RNA-PCR-Derived RNAs -- Identifying Interacting Proteins in an Escherichia coli-Based Two-Hybrid System -- Future Perspectives.
Keywords: Cell biology; Cell Biology