E. coli : Shiga Toxin Methods and Protocols - Philpott, Dana.; Ebel, Frank; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections -- Methods for Detection of STEC in Humans -- Serological Methods for the Detection of STEC Infections -- Detection and Characterization of STEC in Stool Samples Using PCR -- Molecular Typing Methods for STEC -- STEC in the Food Chain -- STEC as a Veterinary Problem -- Cellular Microbiology of STEC Infections -- Analysis of Pathogenicity Islands of STEC -- Generation of Isogenic Deletion Mutants of STEC -- Generation of Monoclonal Antibodies Against Secreted Proteins of STEC -- Microscopic Methods to Study STEC -- Detection and Characterization of EHEC-Hemolysin -- Shiga Toxin Receptor Glycolipid Binding -- Methods for the Purification of Shiga Toxin 1 -- Methods for the Identification of Host Receptors for Shiga Toxin -- Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport -- Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin -- Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways -- Interaction of Shiga Toxin with Endothelial Cells -- Shiga Toxin Interactions with the Intestinal Epithelium -- Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes -- Animal Models for STEC-Mediated Disease -- Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC -- Bovine Escherichia coli O157:H7 Infection Model.
Keywords: Microbiology; Microbiology