Functional Analysis of DNA and Chromatin - Stockert, Juan C.; Espada, Jesús.; Blázquez-Castro, Alfonso; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern -- Using Microchip Gel Electrophoresis to Probe DNA-Drug Binding Interactions -- Identifying Different Types of Chromatin using Giemsa Staining -- Analysis of DNA Damage and Repair by Comet Fluorescence in situ Hybridization (Comet-FISH) -- Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level -- Polarization Microscopy of Extended Chromatin Fibres -- DNA Labeling in Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image Processing Software -- A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation -- Image Analysis of Chromatin Remodelling -- FISH Methods in Cytogenetic Studies -- Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker -- Atomic Force Microscopy for Analyzing Metaphase Chromosomes. Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns -- Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections -- Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage Studies -- Visualization and Interpretation of Eukaryotic DNA Replication Intermediates in vivo by Electron Microscopy -- Combined Bi-dimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replication Intermediates in Human Cells -- Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine (5mC) Immunological Detection -- Methyl-combing-Single Molecule Analysis of DNA Methylation on Stretched DNA Fibers -- Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art -- Hydroxymethylated DNA Immunoprecipitation (hmeDIP) -- Microscale Thermophoresis for the Assessment of Nuclear Protein Binding Affinities -- Analysis of Histone Post-translational Modifications from Nucleolus-associated Chromatin by Mass Spectrometry -- Salt-Urea, Sulfopropyl-Sepharose, and Covalent Chromatography Methods for Histone Isolation and Fractionation -- Chromatin Immunoprecipitation -- Analysis of Chromatin Composition of Repetitive Sequences: the ChIP-chop Assay -- Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae -- Deep Sequencing of Small Chromatin-Associated RNA - Isolation and Library Preparation -- Deep Sequencing of Small Chromatin-Associated RNA - Bioinformatic Analysis.
Keywords: Cell biology; Human genetics; Cell Biology; Human Genetics