Connexin Methods and Protocols - Bruzzone, Roberto; Giaume, Christian; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Tools to Study Connexins -- Investigation of Connexin Gene Expression Patterns by In Situ Hybridization Techniques -- Sodium Dodecyl Sulfate-Freeze-Fracture Immunolabeling of Gap Junctions -- Purification of Gap Junctions -- Culturing of Mammalian Cells Expressing Recombinant Connexins and Two-Dimensional Crystallization of the Isolated Gap Junctions -- Connexins/Connexons -- Biochemical Analysis of Connexon Assembly -- Expression and Imaging of Connexin-GFP Chimeras in Live Mammalian Cells -- Analysis of Connexin Expression in Brain Slices by Single-Cell Reverse Transcriptase Polymerase Chain Reaction -- Use of Retroviruses to Express Connexins -- Spatiotemporal Depletion of Connexins Using Antisense Oligonucleotides -- Transfection and Expression of Exogenous Connexins in Mammalian Cells -- Assays For Function -- Assaying the Molecular Permeability of Connexin Channels -- Applying the Xenopus Oocyte Expression System to the Analysis of Gap Junction Proteins -- Mutagenesis to Study Channel Structure -- Dual Patch Clamp -- Determining Ionic Permeabilities of Gap Junction Channels -- Fluorescence Recovery After Photobleaching -- Capture of Transjunctional Metabolites -- Physiology and Biology of Connexins -- The Study of Connexin Hemichannels (Connexons) in Xenopus Oocytes -- Exploring Hemichannel Permeability In Vitro -- Inducing De Novo Formation of Gap Juntion Channels -- Recording and Analysis of Putative Direct Electrical Interactions in the Mammalian Brain -- Intercellular Calcium Signaling and Flash Photolysis of Caged Compounds -- Biochemical Analysis of Connexin Phosphorylation -- How to Close a Gap Junction Channel.
Keywords: Cell biology; Cell Biology