Cytochrome P450 Protocols - Phillips, Ian R.; Shephard, Elizabeth A.; Ortiz de Montellano, Paul R.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
Bioluminescent Assays for Cytochrome P450 Enzymes. In Vitro Drug-Drug Interaction Assay -- High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening -- The Synthesis, Characterization and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites -- High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators -- Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches -- Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis -- An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins -- Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group -- Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes -- Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles -- Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells -- Screening for Cytochrome P450 Reactivity with a Reporter Enzyme -- High-Throughput Fluorescence Assay of Cytochrome P450 3A4 -- Targeted Protein Capture for Analysis of Electrophile-Protein Adducts -- DNA Shuffling of Cytochrome P450 Enzymes -- Measurement of P450 Difference Spectra Using Intact Cells -- DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production -- P450 oxidoreductase - Genotyping, Expression, Purification of Recombinant Protein and Activity Assessments of Wild-Type and Mutant Protein -- LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450 -- Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-allele) Nomenclature Database -- Simultaneous in vivo Phenotyping of CYP Enzymes -- Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase-I Footprinting -- Isolation of Mouse Hepatocytes -- Highly Efficient SiRNA and Gene Transfer into Hepatocyte-like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies.
Keywords: Proteins .; Protein Science; Protein-Ligand Interactions